Purification of an NADH-(dichlorophenol-indophenol) oxidoreductase from Bacillus stearothermophilus
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چکیده
منابع مشابه
The Molar Extinction Coefficient of 2,6-dichlorophenol Indophenol.
Widely varying values for the molar extinction coefficient of 2.6-dichlorophenoi indophenol have been reported by various workers 1-9. A possible source of this variation may be the different samples of DCIP used, due either ~o the presence of impurities or to the presence of mixtures of the anhydrous and hydrated forms of DCIP sodium salt. However. failure to realise that DCIP is a weak acid w...
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Microbial phytases were applied mainly to animal and human foodstuffs in order to improvemineral bioavailability and food processing. In addition, phytases have potentialbiotechnological application in various other fields, such as environmental protection,aquaculture and agriculture. Bacillus sp. DM12, an isolate from a hot spring, produces phytase,which catalyzes the hydrolysis of phytic acid...
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Solubilization of a reduced nicotinamide adenine dinucleotide (NADH)-2,6 dichlorophenol indophenol (DCIP) oxidoreductase associated with the membrane NADH oxidase system of Bacillus megaterium KM was effected by treatment with 0.2% sodium deoxycholate, 8 m urea, or buffer (pH 9.0) in the presence of ethyl-enediaminetetraacetate. These treatments inactivated membrane NADH oxidase. It was found t...
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The D-alanine carboxypeptidase, the major penicillinbinding component in membranes of Bacillus stearothermophilus, was purified by covalent a5nity chromatography. The enzyme was solubilized in the nonionic detergent Triton X-100, bound to a column of a 6-aminopenicillanic acidSepharose derivative, and eluted upon treatment of the column with neutral hydroxylamine. The enzyme was thermophilic, p...
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Xylanase T-6 is a thermostable alkaline-tolerant enzyme that is produced by Bacillus stearothermophilus T-6. Xylanase T-6 was found to bleach pulp effectively at pH 9 and 65 degrees C and was used successfully on an industrial-scale mill trial. To facilitate the future characterization of the protein via X-ray analysis and protein engineering, it was necessary to overexpress the enzyme in Esche...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1980
ISSN: 0264-6021
DOI: 10.1042/bj1910457